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AICAR certificate of analysis, lot PL-2610-009

Certificate PL-26-00131 reports the release tests for AICAR lot PL-2610-009: 99.67% purity by reverse-phase HPLC and identity confirmed by LC-MS, tested October 5, 2026. The lot number printed on each unit matches this certificate.

Certificate numberPL-26-00131
Lot numberPL-2610-009
CompoundAICAR 50 mg
SequencePurine-precursor nucleoside (AMPK activator); phosphorylated intracellularly to ZMP.
Molecular formulaC9H14N4O5
CAS number2627-69-2
Purity (RP-HPLC)99.67%
Identity (LC-MS)Conforms (258.22 Da) · expected 258.23 Da, found 258.22 Da
MethodRP-HPLC (UV 214 nm) + LC-MS
TestedOctober 5, 2026
Retest byOctober 1, 2028
Tested byManufacturer’s in-house laboratory (not an independent third-party test)
ConclusionConforms

Tests run on this lot

TestMethodSpecificationResult
Identity (mass)LC-MS, ESI positiveTheoretical ± 1.0 Da258.22 Da (PASS)
PurityRP-HPLC, UV 254–306 nm, area%≥ 99.0%99.67% (PASS)
Largest impurityRP-HPLC, area%≤ 1.0%0.15% (RT 7.00) (PASS)
Water contentKarl Fischer≤ 5.0%1.90% (PASS)
Bacterial endotoxinLAL, kinetic≤ 5.0 EU/mg0.419 EU/mg (PASS)
SterilityUSP <71>, 14-dayNo growthNo growth (PASS)

What these results mean

HPLC purity is the share of UV-detectable material that elutes as the target compound; it is not the same as net peptide content, which also accounts for water and counter-ions. Identity means the measured mass matched the mass calculated for the compound. A certificate reports only the tests run on this lot; endotoxin, sterility and contaminant panels are not part of standard lot release. How to read a certificate of analysis.

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Other AICAR lots

  • PL-26-00005, lot PL-2609-005, 99.31%, tested September 19, 2026

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