Retatrutide Pen certificate of analysis, lot PL-2610-173
Certificate PL-26-00286 reports the release tests for Retatrutide Pen lot PL-2610-173: 99.20% purity by reverse-phase HPLC and identity confirmed by LC-MS, tested October 7, 2026. The lot number printed on each unit matches this certificate.
| Certificate number | PL-26-00286 |
|---|---|
| Lot number | PL-2610-173 |
| Compound | Retatrutide Pen 15 mg |
| Sequence | H-Y-Aib-QGTFTSDYSI-[αMeLeu]-LDKK(AEEA-γGlu-C20 diacid)AQ-Aib-AFIEYLLEGGPSSGAPPPS-NH₂ (39 residues; Aib2, α-methyl-Leu13, Aib20; Lys17 acylated with eicosanedioic acid via γ-Glu and AEEA; C-terminal amide) |
| Molecular formula | C221H342N46O68 |
| CAS number | 2381089-83-2 |
| Purity (RP-HPLC) | 99.20% |
| Identity (LC-MS) | Conforms (4,731.06 Da) · expected 4731.33 Da, found 4731.06 Da |
| Method | RP-HPLC (UV 214 nm) + LC-MS |
| Tested | October 7, 2026 |
| Retest by | October 6, 2028 |
| Tested by | Manufacturer’s in-house laboratory (not an independent third-party test) |
| Conclusion | Conforms |
Tests run on this lot
| Test | Method | Specification | Result |
|---|---|---|---|
| Identity (mass) | LC-MS, ESI positive | Theoretical ± 1.0 Da | 4,731.06 Da (PASS) |
| Purity | RP-HPLC, UV 214 nm, area% | ≥ 99.0% | 99.20% (PASS) |
| Largest impurity | RP-HPLC, area% | ≤ 1.0% | 0.37% (RT 19.60) (PASS) |
| Assay (label claim) | Assay vs label claim | 14.25–15.75 mg/mL | 15.22 mg/mL (PASS) |
| Bacterial endotoxin | LAL, kinetic | ≤ 5.0 EU/mg | 0.152 EU/mg (PASS) |
| Sterility | USP <71>, 14-day | No growth | No growth (PASS) |
What these results mean
HPLC purity is the share of UV-detectable material that elutes as the target compound; it is not the same as net peptide content, which also accounts for water and counter-ions. Identity means the measured mass matched the mass calculated for the compound. A certificate reports only the tests run on this lot; endotoxin, sterility and contaminant panels are not part of standard lot release. How to read a certificate of analysis.
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