DSIP Nasal Spray
10 mg of delta sleep-inducing peptide (WAGGDASGE) as a sterile solution in bacteriostatic water, in a metered spray bottle. Filled from a peptide lot tested in the manufacturer’s own laboratory, with a certificate.
| Compound | DSIP Delta sleep-inducing peptide |
|---|---|
| CAS number | 62568-57-4 |
| Molecular formula | C35H48N10O15 |
| Theoretical mass | 848.8 Da |
| Sequence / structure | H-WAGGDASGE-OH (9 residues; free N- and C-termini) |
| Contents | DSIP: 10 mg per bottle (≤ 0.1% w/v) |
| Form | Sterile aqueous solution in bacteriostatic water (0.9% benzyl alcohol), supplied in a metered nasal spray bottle |
| Storage, sealed | Store at 2–8 °C, protected from light; do not freeze |
| Format | Solution in a metered nasal spray bottle |
Sizes and lots
- 10 mg · 1 bottle — $58.99
- 10 mg · Kit of 10 — $537.99
DSIP is delta sleep-inducing peptide, the nonapeptide Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu, named in 1977 for the EEG changes it produced in rabbits. Here it comes as a nasal spray, 10 mg of DSIP dissolved in bacteriostatic water with a preservative and packed in a metered spray bottle. Studies of DSIP itself delivered it into the brain's ventricles or the bloodstream, while the nasal route has a literature of its own on whether molecules reach the brain from the nose.
What is in the bottle
The bottle contains 10 mg of DSIP, about 11.8 µmol, dissolved in sterile bacteriostatic water that carries 0.9% benzyl alcohol to hold back microbial growth. According to the safety data sheet the peptide makes up no more than 0.1% w/v of the solution. It is the nonapeptide sold dry in the vials, H-WAGGDASGE-OH, theoretical mass 848.8 Da. Only the preservative has a hazard classification: benzyl alcohol is harmful by ingestion or inhalation, irritates the eyes and can sensitise skin, while DSIP itself is unclassified.
Refrigerate it at 2 to 8 °C, standing upright with the cap on and out of the light. Freezing is excluded. Why peptide solutions are kept cold and dark is explained in peptide storage and stability.
What the research covers
How DSIP was studied
The peptide was named after experiments in which infusion into the cerebral ventricles of rabbits enhanced delta and spindle EEG activity, an effect its synthetic fragments and analogues did not reproduce1. Later work found DSIP readily degraded in blood: incubated with human or rat blood it released tryptophan-like products, at rates that depended on temperature, time and species2. A peptide cleared that quickly in circulation is one reason a route that bypasses the blood draws interest.
The nasal route
A review by Lochhead and Thorne set out how the nose is built and by which routes a substance on its epithelium might reach the central nervous system while bypassing the blood-brain barrier, citing animal and human work on biologics3. That review concerns large molecules in general. DSIP is a small peptide, and none of the DSIP papers cited here used the nasal route.
What is not established
Frequently asked questions
Is the spray the same DSIP as the vials?
Yes. Vials and spray contain the same nonapeptide, H-WAGGDASGE-OH, at 848.8 Da. In the vials it is a dry powder stored frozen at −20 °C; in the spray, 10 mg is already in bacteriostatic water and is refrigerated, not frozen.
How much DSIP is in a bottle?
Each bottle holds 10 mg, roughly 11.8 µmol, and the safety data sheet caps the concentration at 0.1% w/v. Studies that need to set their own concentration are better served by the 5 mg or 10 mg freeze-dried vials, which state a dry mass.
Has DSIP been studied by the nasal route?
Not in any paper cited on this page. The original rabbit experiments infused DSIP into the brain's ventricles, and the nasal delivery review covers biologics in general, so there are no findings specific to this format to point to.
References
- Schoenenberger GA, Monnier M. Characterization of a delta-electroencephalogram (-sleep)-inducing peptide. Proc Natl Acad Sci U S A. 1977;74(3):1282-6. DOI · PubMed
- Graf MV, Saegesser B, Schoenenberger GA. Degradation and aggregation of delta sleep-inducing peptide (DSIP) and two analogs in plasma and serum. Peptides. 1987;8(4):599-603. DOI · PubMed
- Lochhead JJ, Thorne RG. Intranasal delivery of biologics to the central nervous system. Adv Drug Deliv Rev. 2012;64(7):614-28. DOI · PubMed
For laboratory research use only. Not for human or veterinary consumption. Products have not been evaluated by the U.S. Food and Drug Administration and are not intended to diagnose, treat, cure or prevent any disease.