Testagen Pen
10 mg of the Khavinson tetrapeptide in 3 mL of bacteriostatic water at 0.33% w/v. Filled from a peptide lot tested in the manufacturer’s own laboratory, with a certificate.
| Compound | Testagen Lys-Glu-Asp-Gly (KEDG); Khavinson testicular peptide bioregulator |
|---|---|
| CAS number | Not assigned |
| Molecular formula | C17H29N5O9 |
| Theoretical mass | 447.4 Da |
| Sequence / structure | H-KEDG-OH (4 residues; free N- and C-termini) |
| Contents | Testagen: 10 mg per 3 mL pen (0.33% w/v) |
| Form | Sterile aqueous solution in bacteriostatic water (0.9% benzyl alcohol), supplied in a 3 mL glass cartridge inside a preloaded autoinjector pen fitted with a needle |
| Storage, sealed | Store at 2–8 °C in the original carton, protected from light |
| Format | Solution in a 3 mL pre-filled pen |
Sizes and lots
- 10 mg · 1 pen — $68.99
- 10 mg · Kit of 10 — $613.32
Testagen, the Khavinson tetrapeptide Lys-Glu-Asp-Gly (KEDG), is supplied in this listing as a preloaded pen with 10 mg of peptide dissolved in a 3 mL cartridge, half the amount in the 20 mg vial and ready in solution. Studies that name the KEDG sequence are few: pituitary and thyroid work in chickens and hens, and cell uptake in HeLa cells, mostly published in Russian journals. None of them examined the testis.
What is in the pen
Each pen holds a 3 mL glass cartridge of sterile aqueous solution with Testagen at 0.33% w/v, 10 mg per pen, which is about 22 µmol at the certificate's theoretical mass of 447.4 Da. The solvent is bacteriostatic water with 0.9% benzyl alcohol as an antimicrobial preservative. The safety data sheet does not classify the peptide; benzyl alcohol is listed as harmful if swallowed or inhaled, a serious eye irritant and a skin sensitiser.
Keep the pen at 2 to 8 °C in its original carton, protected from light, and capped once in use. Do not let it freeze, and discard it if the solution has frozen, discoloured or turned cloudy. The freeze-dried Testagen 20 mg vial holds twice the amount as dry powder, and peptide pen vs vial compares the formats.
What the research covers
According to Kuznik and colleagues, the sequence behind Testagen was synthesised from the amino acid composition of anterior pituitary extracts1. In their 2011 study, KEDG and the related Ala-Glu-Asp-Gly reduced the hormonal and thyroid changes that followed removal of the pituitary in young chickens and old hens, with a weaker effect on hormones in the old birds1. Fluorescently labelled Testagen has also been followed into the cytoplasm, nucleus and nucleolus of HeLa cells2. Neither the bird nor the cell work measured anything in mammalian tissue. Both papers appeared in Russian journals published in English, and both concern peptides from the bioregulator programme.
Those results feed the developers' broader view that short peptides regulate genes by acting on DNA and histones3, a model that the papers cited here do not test outside the programme's own laboratories. They include no measurement in testicular tissue, and none of the studies used Testagen in solution or looked at how long it stays intact in bacteriostatic water, so the pen's storage limits come from its safety data sheet. The Testagen 20 mg vial page has the full research summary, and the Testagen guide covers the programme's account of the compound.
Frequently asked questions
How does the pen differ from the vial and the nasal spray?
All three hold the same KEDG tetrapeptide. The vial has 20 mg of dry powder kept at −20 °C. The pen has 10 mg dissolved in 3 mL of bacteriostatic water, and the nasal spray bottle has 20 mg in solution; both solutions are stored at 2 to 8 °C and never frozen.
Do the published KEDG studies apply to the pen?
Only as background on the peptide. The studies used birds and cultured cells and say nothing about Testagen's stability in bacteriostatic water. For the pen, the storage conditions on the safety data sheet are the ones that apply.
References
- Kuznik BI, Pateyuk AV, Rusaeva NS, et al. Effects of peptides Lys-Glu-Asp-Gly and Ala-Glu-Asp-Gly on hormonal activity and structure of the thyroid gland in hypophysectomized young chickens and old hens. Bull Exp Biol Med. 2011;150(4):495-9. DOI · PubMed
- Fedoreyeva LI, Kireev II, Khavinson VKh, et al. Penetration of short fluorescence-labeled peptides into the nucleus in HeLa cells and in vitro specific interaction of the peptides with deoxyribooligonucleotides and DNA. Biochemistry (Mosc). 2011;76(11):1210-9. DOI · PubMed
- Khavinson VK, Popovich IG, Linkova NS, et al. Peptide Regulation of Gene Expression: A Systematic Review. Molecules. 2021;26(22). DOI · PubMed
For laboratory research use only. Not for human or veterinary consumption. Products have not been evaluated by the U.S. Food and Drug Administration and are not intended to diagnose, treat, cure or prevent any disease.